Fig. 8

Overexpression of TKT promotes the proliferation, metastasis, lactic acid and glucose uptake in GC cells. A, B The proliferation was detected by CCK-8 and clone assay in each group. C The proliferation was detected by EDU in each group. D The changes of cell cycle were detected by flow cytometry in each group. E The changes of cycle-critical proteins were detected by WB. F Annexin V-APC/PI and WB were used to detect apoptosis and apoptosis-related proteins. G, H The cell migration and invasion abilities were detected by wound healing and transwell assays. I, J Changes of EMT and PI3K/AKT signaling pathway related proteins were detected by WB. K, L Changes of lactic acid and total glucose in each group. M Subcutaneous tumorigenesis was performed in nude mice in each group. N The expressions of Ki67, MND1, E-cadherin, N-cadherin and Vimentin were detected by IHC. O Schematic diagram of the role of FOXA1/MND1/TKT signaling axis in regulating GC progression and oxaliplatin sensitivity via PI3K/AKT signaling pathway (*P-value < 0.05, **P-value < 0.01, ***P-value < 0.001). All figures represent mean ± SD from three independent experiments