Fig. 2
From: Synergistic effect of YOD1 and USP21 on the Hippo signaling pathway
Ubiquitinated and regulation of YOD1 and USP21 by the UPS. A A diagram of lysine mutant constructs of ubiquitin. B A ubiquitination assay was performed on Myc-YOD1 by co-transfecting HEK293T cells with Myc-YOD1 and HA-Ub. C HEK293T cells were transfected with Myc-YOD1 and HA-Ub along with seven lysine mutant forms of ubiquitin (HA-Ub+K6, HA-Ub+K11, HA-Ub+K27, HA-Ub+K29, HA-Ub+K33, HA-Ub+K48, and HA-Ub+K63) to perform a more detailed analysis of Myc-YOD1. IP was conducted with an anti-Myc antibody, and the ubiquitination level was detected with an anti-HA antibody. D Flag-USP21 was co-transfected with HA-Ub into HEK293T cells the ubiquitination assay. E A similar ubiquitination assay was conducted for Flag-USP21, with HEK293T cells being transfected with Myc-YOD1 and HA-Ub, along with its seven lysine mutant forms of ubiquitin