Fig. 3
From: Synergistic effect of YOD1 and USP21 on the Hippo signaling pathway
YOD1 and USP21 exhibit reciprocal deubiquitination activity on polyubiquitin chains. A The deubiquitination assay for Myc-YOD1: HEK293T cells were transfected with Myc-YOD1, Flag-USP21, and Flag-USP21 (C221S) along with HA-tagged Ub. Myc-YOD1 was shown to deubiquitinate K27, K29, K48, and K63-linked chains on USP21. B The deubiquitination assay for Flag-USP21: HEK293T cells were transfected with Flag-USP21, Myc-YOD1, and Myc-YOD1 (C160S) along with HA-tagged Ub. C The deubiquitination assay for Myc-YOD1: HEK293T cells were transfected with Myc-YOD1, Flag-USP21 and HA tagged-ubiquitin constructs with specific ubiquitin mutants. D The deubiquitination assay for Flag-USP21: Flag-USP21 was co-transfected with Myc-YOD1 and HA tagged-specific mutant ubiquitin constructs into HEK293T cells