Fig. 4

YOD1 upregulates USP21 protein stability, but not vice versa. A HEK293T cells were transfected with increasing amounts of Flag-USP21 and Myc-YOD1. Western blotting was used to assess the expression level of Flag-USP21. The expression of Flag-USP21 was quantified in at three independent experiments using one-way ANOVA analysis and the data are presented as means ± SEM (with error bars) (***p < 0.001). B HeLa and A549 cells were transfected with increasing amounts of Flag-USP21 and Myc-YOD1. Western blotting was used to assess the expression level of Flag-USP21. C HEK293T cells were transfected with increasing amounts of Myc-YOD1 and Flag-USP21. Western blotting was used to assess the expression level of Myc-YOD1. The expression of Myc-YOD1 was quantified in at three independent experiments using one-way ANOVA and the data are presented as means ± SEM (with error bars) (NS not significant). D HeLa and A549 cells were transfected with increasing amounts of Myc-YOD1 and Flag-USP21. Western blotting was used to assess the expression level of Myc-YOD1