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Fig. 3 | Cancer Cell International

Fig. 3

From: The MYBL2–CCL2 axis promotes tumor progression and resistance to anti-PD-1 therapy in ovarian cancer by inducing immunosuppressive macrophages

Fig. 3

MYBL2-CCL2 axis promoted recruitment and M2 polarization of macrophages in vitro. (A) Chemotactic migration assays of macrophages using the supernatant of SKOV3 or A2780 cells. (B-C) Flow cytometry analysis of CD204 + cells and CD206 + cells in PMA-stimulated THP-1 cells treated with the supernatant of SKOV3 and A2780 cells for 48 h. (D) Association between the expression of MYBL2 and the expression of CD206 by immunohistochemistry. (E) Chemotactic migration assays of macrophages using the supernatant of SKOV3 cells. (F) Flow cytometry analysis of CD206 + proportion and expression in PMA-stimulated THP-1 cells treated with the supernatant of SKOV3 cells for 48 h. (G) Flow cytometry analysis of CD206 expression in bone marrow-derived macrophages (BMDMs) treated with the supernatant of ID8 cells for 24 h. (H) Quantitative PCR analysis of Arg-1, IL-1β, IL-12a, and CD86 in BMDMs treated with the supernatant of ID8 cells for 24 h. (I) CD206 and CD163 immunohistochemical staining and quantification in the tumors of mice

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Cancer Cell International

ISSN: 1475-2867

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