Fig. 1

Characterization of the EVs isolated from the MDA-MB-231 cells conditioned media. Serum-starved triple-negative breast cancer-derived MDA-MB-231 cells were cultured for 48 h in the absence or presence of 30 µM EGCG. Conditioned media was next collected, concentrated, and extracellular vesicles (EVs) isolated as described in the Methods section. Dynamic light scattering particle size analysis of the A EVs and B EGCG-EVs distribution of the particles by number (upper panels) and by intensity (lower panels) of the refracted light. A representative experiment out of four is presented. C Immunoblotting of the exosomes enriched proteins CD9, CD63 and CD81, and of the negative marker BIP in MDA-MB-231 cell lysate and EVs lysate. D Gating strategy of the flow cytometry experiment performed to assess MemGlow-488 labelled EVs interaction with hADMSC. Merged histogram was obtained by the measurement by flow cytometry of the untreated cells (black lines) and the cells incubated with stained-EVs (red lines). A representative experiment out of two is presented. E Representative microscopy images of hADMSC incubated for two hours with the MDA-MB-231 cells-derived EVs labelled with MemGlow-488. A representative experiment out of three is presented (scale bar is 20 µm). F Relative cell migration rate of hADMSC treated with EVs (closed circle) or basal media (BM, open circle) in response to basal media supplemented with 1% FBS. Migration experiments were performed three times in quadruplicate. Diameter in nanometers (d.nm), polydispersity index (Pdl), intensity weighted mean hydrodynamic size of the particles (Z-Average). Statistically significant differences were determined by the non-parametric comparison test Mann–Whitney, * p < 0.05