Fig. 3

Signalling cascades triggered by the EVs. The hADMSC were incubated for one hour in basal media (BM), EVs, or EGCG-EVs using a Cell:EVs ratio of 1:0,5 (#:#). Cells were next lysed as described in the Methods section for Western blotting analysis. A phospho-kinase array was used to detect pathways activation states. A Immunoblotting results (1, p38a; 2, STAT5a/b; 3, p53; 4, Chk-2; 5, c-Jun; 6, Akt 1/2/3; 7, GSK-3b), and B Densitometric analysis of the highlighted immunoreactive spots performed using the ImageJ software. C Validation of the phosphorylated and total states of GSK-3β (Ser9) and AKT (Ser473) by immunoblotting. A representative experiment out of two is presented. D Ratios of the phosphorylated/total forms of AKT and GSK-3β resulted from the densitometric analysis performed with ImageJ. Mitogen-activated protein kinases (p38); signal transducer and activator of transcription 5A/B (STAT5a/b); Checkpoint kinase-2 (Chk-2); c-Jun N-terminal kinases JNK (c-Jun); protein kinase B signaling pathway (AKT); glycogen synthase kinase-3 (GSK-3); tumor protein 53 (p53)