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Fig. 4 | Cancer Cell International

Fig. 4

From: EGCG inhibits the inflammation and senescence inducing properties of MDA-MB-231 triple-negative breast cancer (TNBC) cells-derived extracellular vesicles in human adipose-derived mesenchymal stem cells

Fig. 4

Induction of a pro-inflammatory molecular signature by the MDA-MB-231 cells-derived EVs. The hADMSC were incubated for 24 h in Basal Media (BM, Control), EVs (white bars) or EGCG-EVs (black bars) with a Cell: EVs ratio of 1:0,5. Next, total RNA was isolated, and cDNA was synthesized. Gene expression levels were determined by qPCR using a Human Inflammatory Cytokine and Receptors RT2-Profiler gene array kit. Densitometric analysis was performed using the ImageJ software. A The fold change (FC) expression of genes related to the cancer-associated adipocyte (CAA) phenotype. Validation of the arrays results for CAA genes was performed in two independent experiments. B Immunoblotting of interleukin-6 (IL-6) and tubulin (10 µg protein/well). Immunoblotting is representative of three experiments. C FC of selected genes from the array to highlight the modulatory effect of the EGCG-EVs

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Cancer Cell International

ISSN: 1475-2867

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