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Fig. 6 | Cancer Cell International

Fig. 6

From: EGCG inhibits the inflammation and senescence inducing properties of MDA-MB-231 triple-negative breast cancer (TNBC) cells-derived extracellular vesicles in human adipose-derived mesenchymal stem cells

Fig. 6

EGCG reduces the mitochondrial content within the EVs. Serum-starved MDA-MB-231 cells were cultured for 48 h in the presence or absence of 30 µM EGCG. EVs were isolated, stained with anti-CD44-FITC and MitoTracker Deep Red (MTR), and analyzed by flow cytometry. Four independent experiments were spaced in time from cell passage 3 to 8. The dots represent the mean of the counting, and results from the same experimental day were connected with a line in graphs A-C. Paired t-test was performed; **P < 0.01. A The total number of CD44+/MTR− microparticles (MPs) detected in EVs or EGCG-EVs. B Quantification of the mitochondria-containing particles (CD44+/MTR+, mitoMPs) in the EVs or EGCG-EVs. Four independent experiments were performed. C Citrate synthase activity was measured in particles isolated from the conditioned media as described in the Methods section. D Dot plot resulting from the flow cytometry analysis detecting the presence of mitochondria delivered by the EVs in hADMSC after incubation with basal media (BM, negative control), mitoTracker-labelled EVs (MTR-EVs), or mitoTracker-labelled ECGC-EVs (MTR-EGCG-EVs). E Mean of the fluorescence intensity of the EVs or EGCG-EVs -delivered mitochondria within hADMSC. F The percent of hADMSC positive for the presence of mitochondria delivered by the EVs or EGCG-EVs

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Cancer Cell International

ISSN: 1475-2867

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