Fig. 7

Transcriptomic analysis of the influence of low oxygen tension and EGCG in loading the MDA-MB-231-derived EVs. A Principal Component Analysis (PCA) of the top 500 differential expressed genes (DEG) identified in the samples. B Venn diagram showing the number of DEG detected with a log2 FC ≥|2| in the EVs obtained by adding 30 µM EGCG at different oxygen tensions. The analysis was performed by comparing the EGCG-EVs obtained in hypoxia (EV_HE, C3) vs EGCG-EVs obtained in normoxia (EV_NE, C2). Genes with a significant or non-significant value were coloured in red and grey respectively. C) Robust k-means clustering visualized as a heatmap of the individual samples and their DEG with a log2 FC ≥|2| and p-adjusted value < 0.05. D Gene ontology (GO)-SLIM PANTHER analysis showing the biological process that involves genes downregulated by HE (cluster 1, heat map). E List of all biological processes downregulated by HE. F GO-SLIM PANTHER analysis of the downregulated genes in cluster 1 of the heatmap, showing their protein class. Fisher’s exact test and the false discovery rate (FDR) correction were used during the GO-SLIM analysis