Fig. 1

IL-6 upregulated the expression of KDM2A in mammary fibroblasts by activating STAT3. A Overexpression of constitutively active STAT3 by pSTAT3 plasmid increased the expression of KDM2A in RMF-EG cells. The protein and mRNA levels of KDM2A were analyzed by Western blotting analysis and real-time PCR, respectively. B RMF-EG cells were transfected with dominant-negative STAT3 plasmids and then treated with or without 10 ng/ml IL-6 for 48 h. The protein and mRNA levels of KDM2A were analyzed by Western blotting analysis and real-time PCR, respectively. The pcDNA3.1 vector was used as the control vector. C RMF-EG cells were incubated with HS-578 T CM for 48 h in the presence or absence of 5 μM stattic. The protein and mRNA levels of KDM2A were analyzed by Western blotting analysis and real-time PCR, respectively. IL-6 expression in serum-free medium (SF) or HS-578 T CM was analyzed by Western blotting analysis. D RMF-EG cell was treated with 20 ng/ml IL-6 for 48 h in the presence or absence of 5 μM stattic. The protein and mRNA levels of KDM2A expression were analyzed by Western blotting analysis and real-time PCR, respectively. Differences were found to be statistically significant at * p < 0.05, ** p < 0.01, and *** p < 0.001