Fig. 4

KDM2A-expressing mammary fibroblasts promoted M2 macrophage polarization. A PMA-pretreated THP-1 cells were co-cultured with RMF-EG cells or KDM2A-expressing RMF-EG cells (K2A-1), and the relative expression of M2-macrophage markers CCL2, IL-10, and VEGF-A were determined by real-time PCR. B Relative mRNA levels of tumor-associated fibroblast surface marker CD206 and CD163 in THP-1 cells co-cultured with RMF-EG or KDM2A-expressing RMF-EG (K2A-1) cells were determined by real-time PCR. C The relative expression levels of CD206 and CD163 on the cell surface of THP-1 cells co-cultured with RMF-EG or KDM2A-expressing RMF-EG (K2A-1) cells were determined and quantified by flow cytometry. Each experiment was performed in triplicate and repeated three times independently. Differences were found to be statistically significant at * p < 0.05, ** p < 0.01, and *** p < 0.001