Fig. 2

Biological impact of RKIP KO in GIST-T1 cell line. (A): Western Blot analysis of RKIP expression in GIST-T1 negative control (NC) and RKIP Knockout cells (KO). α-tubulin was used as the loading control. (B): Matrigel invasion assay in both GIST-T1 negative control and RKIP KO cells after 24 h at baseline. The results were expressed as the mean number of invading cells ± SD. (C): Cell migration of RKIP control and RKIP KO cells using Wound Healing Assay after 24 h, and 48 h at baseline. The results were expressed in relation to zero time point (considered as 0% of migration) and considering the mean percentage of migration ± SD. Both invasion and migration assays were done in triplicate and expressed as the mean of three independent experiments. Significance was considered at p < 0.05 (*)