Fig. 5

COL3A1 is a novel putative target of RKIP in gastrointestinal tract tumors. (A): Schematic representation of the differentially expressed genes found at the transcriptomic and proteomic analysis. (B): Using TCGA data, available at CBioportal (www.cbioportal.org), it was used the log RNA seq RPKM data from 3 TCGA PanCancer Atlas studies (Esophageal, colorectal, and stomach adenocarcinoma), to determine the correlation levels between RKIP (PEBP1) and the 6 commonly found altered molecules (A). The correlation plots and the Spearman correlation coefficients (Suppl. Table S2) are graphically represented as a heatmap, where PEBP1 is classified as positively (p > 0; dark to green) or negatively (p < 0; dark to red) correlated with the represented proteins. (C): Functional protein association network was done in STRING tool. (D): Western blot analysis of COL3A1 and RKIP expression in RKIP KO (Knockout) and NC (Negative Control) cells. GAPDH was used as a loading control, and COL3A1 expression was quantified as a ratio with GAPDH. The graph of relative protein quantification is expressed by the mean ratio with GAPDH, from two independent experiments. Significance was considered at p < 0.05 (*)