Fig. 6
Effect of RKIP on GIST-T1 cell line response to Imatinib and Regorafenib. Representative dose-response curves of control and KO cells treated with Imatinib (A) and Regorafenib (B). The data are expressed as the percentage ± SD of viable cells, relative to the DMSO alone (considered 100% viability), and calculated as the mean of three independent assays, done in triplicate. (C): 3D in vitro proliferation model for control and RKIP KO cells, treated for 72 h with Imatinib at IC50 (0.015 µM) and at 1 µM concentration (D). The results show the mean percentage ± SD of viable cells in 3D spheres after imatinib treatment, relative to untreated cells (considered 100% viability - dot line). The experiment was done in triplicate and expressed as the mean of three independent assays. Significance was considered at p < 0.05 (*)