Fig. 7

Imatinib-induced apoptosis is abolished by the RKIP edition in GIST cells. (A): GST-T1 cells, negative control, and RKIP KO cells were treated with indicated concentrations of imatinib for 24 h. Expression levels of apoptosis-associated proteins such as PARP, caspases 7 and 3, and BAX were assessed by performing a western blot. β-Actin was used as a loading control and the dotted line is indicating the proteins cleaved bands (B): Western blot quantification by bands densitometry, expressed as absolute quantification for cleaved PARP and Caspase-7 (CASP7), and as a ratio with β-Actin for BAX. The graphs are expressed as the mean of two independent experiments