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Fig. 10 | Cancer Cell International

Fig. 10

From: Systematic analysis of integrated bioinformatics to identify upregulated THBS2 expression in colorectal cancer cells inhibiting tumour immunity through the HIF1A/Lactic Acid/GPR132 pathway

Fig. 10

THBS2 influences M2 polarisation of macrophages through GPR132. A Collection of conditioned medium from THBS2-overexpressing CT26 cells and its addition to RAW264.7 cells. Flow cytometric analysis of CD163 and CD206 expression in cells after 24 h; B Centrifugation of conditioned medium from CT26 cells to isolate high-molecular-weight proteins and the addition of the medium to RAW264.7 cells. Flow cytometric analysis of CD163 and CD206 expression in cells after 24 h; C Addition of lactate to RAW264.7 cell culture medium. Flow cytometric analysis of CD163 and CD206 expression in cells after 24 h. D Flow cytometric analysis of GPR132 expression in peripheral blood mononuclear cells from GPR132-knockout (KO) mice; E Collection of conditioned medium from THBS2-overexpressing CT26 cells and its addition to macrophages from GPR132-KO mice. Flow cytometric analysis of CD163 and CD206 expression in cells after 24 h

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Cancer Cell International

ISSN: 1475-2867

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