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Fig. 9 | Cancer Cell International

Fig. 9

From: Systematic analysis of integrated bioinformatics to identify upregulated THBS2 expression in colorectal cancer cells inhibiting tumour immunity through the HIF1A/Lactic Acid/GPR132 pathway

Fig. 9

THBS2 influences lactate metabolism in CT26 cells through HIF1. A Representative images from three independent western blotting experiments showing the overexpression of THBS2 in CT26 cells. B Immunofluorescence staining of HIF1 (green) and nuclei (blue) in CT26 cells overexpressing THBS2. Representative images from three independent experiments are shown. C Detection of HIF1 expression in the nucleus and cytoplasm of CT26 cells overexpressing THBS2. D Measurement of extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) in CT26 cells overexpressing THBS2 on a Seahorse analyser. E Assessment of lactate metabolism in CT26 cells overexpressing THBS2 (n = 3; *** indicates p < 0.001 compared with the vector group). F Detection of downstream transcriptional genes of HIF1 via PCR (n = 3; *** indicates p < 0.001 compared with the vector group). G Representative images of western blotting showing the knockdown of HIF1 in THBS2-overexpressing CT26 cells. Representative images from three independent experiments are shown. H Evaluation of lactate metabolism after THBS2 overexpression and HIF1 knockdown in CT26 cells (n = 3; ns indicates no significant difference compared with the vector group)

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Cancer Cell International

ISSN: 1475-2867

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