Fig. 8

EVs prepared from DLBCL dogs diminished the anti-tumor activity of CD8 + T cells. A CD8 + T cells were co-incubated with 100 and 200 µg/ml DLBCL-derived EVs for 48 h, and the apoptotic rate (Annexin V + /PI +) was analyzed by flow cytometry. B Percentage and corresponding quantification of mean fluorescence intensity (MFI) of IFN-r; C CLTA-4 expression in CD8 + T cells after incubation of DLBCL EVs. D Gene expression of immune-regulatory molecules was detected in CD8 + T cells treated with DLBCL EVs at 24 h. Data are obtained from the EVs purified from the blood of 6 DLBCL dogs. Bar graphs reflect mean ± SEM (n = 6) analyzed by two-way ANOVA with Tukey's post hoc or Mann–Whitney U test (quantitative PCR). ***, P < 0.001; ****, P < 0.0001