Fig. 2

The proliferation of T cells cocultured with DCs. A Flow cytometry histogram of CFSE dilution from one representative experiment are shown. CFSE-labeled T cells were stimulated by IL-2, immature DCs and LP-loaded mature DCs and without stimulation as a control after 5 days. The proliferation of CFSE-labeled T cells was determined using flow cytometry. B Quantitative data from the division index (average division numbers that a cell in the original population had gone through; the average also takes into account the undivided cells) for each group from four independent experiments showing significance between different groups and a higher percentage for LP-loaded mDCs. C Colonies generated by each group of experiments. Bar: 200 μm and applied to all images. D Size comparison of generated colonies indicated a significant increase in colony diameter from LP-loaded mDCs compared with the other groups. All groups showed significant differences. A Paired t test was used to compare the differences between two groups. Data are presented as mean ± SD. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001. imDC: immature DCs, mDCs: LP-loaded mature DCs