Fig. 4

The effect of TSTA3 overexpression on the proliferative and metastatic ability of LUSC in vivo. A Tumor tissues in a subcutaneous tumor xenograft model (n = 6). B, C Tumor growth curves (B) and tumor weight (C) of subcutaneous tumor xenograft model (n = 6). D Immunohistochemistry detects the expression of Ki67 in xenograft tumor tissues of xenograft model mice. Scale bar, 50 μm. E Representative images of liver tissues of the NC group and TSTA3-OE group. F The number of liver metastatic nodules in NC group and TSTA3-OE group. G HE staining of liver tissues in NC group and TSTA3-OE group. Scale bar, 100 μm. H Survival curves of NC group and TSTA3-OE group. I Volcanic map of RNA sequencing in TSTA3 overexpression and negative control group H226 cells, with 47 genes up-regulated and 221 genes down-regulated in transcriptome data with fold change > 0.585 and P < 0.05. J Bubble diagram of KEGG-based enrichment analysis was performed for those 221 genes down-regulated with significant differences. **** P < 0.0001; *** P < 0.001; ** P < 0.01