Fig. 3

Cell viability and cell cycle progression of PFKFB3-or PFKFB4-silenced SAS and TW2.6 cells. The mRNA and protein levels of PFKFB3 or PFKFB4 were evaluated by A RT-qPCR and B Western blotting in PFKFB3- or PFKFB4-silenced cells. C Cell viability of PFKFB3-or PFKFB4-knockeddowned cells was analyzed by CellTiter-Glo assay. D Cell cycle progression of PFKFB3-silenced cells was analyzed by flow cytometry. E Cell cycle-related proteins in PFKFB3-silenced cells were analyzed by Western blotting. The 10 nM scrambled siRNA (siCtrl) or siRNA against PFKFB3 or PFKFB4 (siPFKFB3 or siPFKFB4) were transfected into cells for 72 h. All data were represented as the average ± SD from 3 independent experiments. The significant differences between the scrambled control and knocked down cells were indicated as ^* p < 0.05, ^** p < 0.01, and ^*** p < 0.001