Fig. 4

Cell migration and expression of EMT-related markers in PFKFB3- or PFKFB4-silenced SAS and TW2.6 cells. The cell migration of A PFKFB3-silenced and B PFKFB4-silenced cells was measured by the wound-healing assay. C Expression of EMT markers (Slug and E-cad) in PFKFB3-silenced cells were measured by qRT-PCR. The 10 nM scrambled siRNA (siCtrl) or siRNA against PFKFB3 or PFKFB4 (siPFKFB3 or siPFKFB4) were transfected into cells for 72 h. All data were represented as the average ± SD from 3 independent experiments. The significant differences between the scrambled control and knocked down cells were indicated as ^* p < 0.05, ^** p < 0.01, and ^*** p < 0.001