Fig. 3
From: HADHA promotes ovarian cancer outgrowth via up-regulating CDK1
CDK1 is a downstream target of HADHA. (A) The levels of HADHA, CDK1, PCNA, CAT, RAN and PGD were detected in HADHA-depleted HO-8910 and SK-OV-3 cells. (B) The endogenous relationship between HADHA and CDK1. (C) The half-life of CDK1 was analyzed in HO-8910 and SK-OV-3 cells with/without HADHA depletion. (D) The expression of CDK1 was detected in HADHA-depleted HO-8910 and SK-OV-3 cells after MG132 treatment. (D) The ubiquitination level of CDK1 was detected in immunoprecipitates of CDK1 collected from HADHA-depleted HO-8910 and SK-OV-3 cells