Fig. 2

OS-derived exosomal linc00881 promotes OS lung migration and induces lung fibroblasts activation. A. RNA sequencing in a heatmap. B, C. Scatter plots of lncRNA expression in different groups. D. Relative expression of linc00881 in HFL-1 cells simulated by two OS exosomes. E. qRT-PCR detection of the expression linc00881 in exosomes with inhibited or overexpressed linc00881 in MG63 or HOS cells. F. Schematic diagram of the cell co-culture in vitro model. G–J. Representative images of MG63 (G) and HOS (I) cells migrated to HFL-1 cells with overexpressed or inhibited linc00881 in MG63 or HOS exosomes by transwell assay and the corresponding quantitative analysis (H, J). K–N. Representative images of MG63 (K) and HOS (M) cells migrated to HFL-1 cells with or without an inhibited expression of linc00881 in HFL-1 cells by transwell assay and the corresponding quantitative analysis (L, N). O, P. qRT-PCR detection of the relative expression of IL-1β, IL-6, IL-8, and α-SMA in HFL-1 cells with inhibited expression of linc00881 in MG63 or HOS exosomes. (*p < 0.05; **p < 0.01; *** p < 0.0001)