Fig. 4

miR-29c-3p directly regulates the expression of MMP2 in lung fibroblasts cells. A. Duplex structure of MMP2 3′-UTR (Untranslated Region) and miR-29c-3p. B. The relative expression of MMP2 was detected at the mRNA level by qRT-PCR using a control or miR-125a-5p probe to perform the pull-down assay. C. The miR-29c-3p expression was detected via qRT-PCR after the control/miR-29c-3p mimic or control/miR-29c-3p inhibitor was transfected into HFL-1 cells. D–F. Representative images (D) and quantitative analysis (E, F) of Western blotting analysis and qRT-PCR of MMP2 expression in HFL-1 cells after transfected as described in C. G, H. Representative images (G) and quantitative analysis (H) of Western blotting analysis of MMP2 expression in HFL-1 cells transfected as follows: a. control mimic and control vector; b. control mimic and the miR-29c-3p-overexpressed plasmid; c. miR-29c-3p mimic and control vector; d. miR-29c-3p mimic and MMP2 vector. I–L. Representative images (I MG63, K HOS) and quantitative analysis (J, L) of transwell assay of MG63 and HOS cells migrated to HFL-1 cells after transfected as described in G. (*p < 0.05; **p < 0.01; *** p < 0.0001)