Fig. 5

Exosomal Linc00881 regulates the expression of MMP2 in lung fibroblasts by sponging miR-29c-3p. A, B. Representative images (A) of Western blotting analysis of MMP2 expression in HFL-1 cells treated with blank, MG63, or HOS exosomes and the corresponding quantitative analysis (B). C. Relative miR-29c-3p concentration was detected in HFL-1 cells simulated by the two types of OS-derived exosomes. D-F. Representative images (D) of Western blotting analysis of MMP2 expression in HFL-1 cells transfected with linc00881 siRNA or vector and the corresponding quantitative analysis (E, F). G. qRT-PCR detection of the expression of linc00881 in HFL-1 cells transfected with linc00881 siRNA or vector. H. qRT-PCR detection of the expression of miR-29c-3p in HFL-1 cells transfected as described in B. I–K. Representative images (I) of Western blotting analysis of MMP2 expression in HFL-1 cells transfected with miR-29c-3p mimic and stimulated with MG63 or HOS cells-derived exosomes, and the corresponding quantitative analysis (J, K). L–O. qRT-PCR detection of the relative expression of linc00881 or miR-29c-3p in HFL-1 cells treated in I. (*p < 0.05; **p < 0.01; *** p < 0.0001)