Fig. 2

Gene set enrichment and exomiRNAs target-gene analysis of deregulated exo-miRNAs in PCa cells vs RWPE-1 cell line. a ExomiRNAs differentially expressed in PCa cell lines (PC-3 and LNCaP) versus healthy cell line (RWPE-1). The Venn diagram shows the number of exomiRNAs differentially expressed in LNCaP (blue circle), PC-3 (red circle) and in both regarding the normal healthy prostate epithelial cell line (RWPE-1) (purple circle). For each category, the exomiRNAs upregulated (indicated in green) and downregulated (indicated in red) are indicated in the diagram. (*) hsa-miR-663b is overexpressed in PC-3 and under-expressed in LNCaP relative to RWPE-1. b Reactome pathway enrichment analysis list of significant pathways affected by downregulated exomiRNAs in PCa cells and putative implicated target genes. C STRING analysis of protein–protein interaction network of the exomiRNAs target genes TNRC6B, AGO1 and TNRC6A. Coloured circle predicted functional protein partners. Different colours lines represent different evidence of a connection between proteins (Black—Co-expression, pink—experimental evidence, turquoise—database evidence)