Fig. 4

Exam the downstream targets of ALKBH5 in Gastric Cancer. (A) Extraction of MeRIP-seq data comparing the distribution of genes with differential expression and degree of m6a modification in AGS cells with those in the control group. (B) HOMER highest shared motifs with m 6 A-seq peaks in AGS cells with or without ALKBH5 knockdown. (C) In the RNA-seq dataset (m 6 A-seq input library) we selected 265 genes that showed more than 8-fold numerical differences. In shALKBH5 there are 5,180 unique m 6 A peaks for 1591 genes. The two were intersected and 45 genes were identified. (D) RT-qPCR was used to detect the expression of the 16 genes screened out in ALKBH5 knockdown versus control cells (Data are expressed as mean ± SD. * p < 0.05), and assays were repeated 3 times biologically. (E) MeRIP-seq data comparing the expression of the m 6 A-seq peak at the CHAC1 locus in ALKBH5 knockdown and control cells