Fig. 4

EV differential proteins HSP60 were analyzed in H. pylori infected cells and GC tissues. A, B EV-derived HSP60 in H. pylori uninfected group and H. pylori infected group (Hp vs control) were detected by western blotting. B The relative expression of HSP60 was semiquantified. C–F The expression levels of HSP60 in H. pylori infected (C, D) GES-1 and (E, F) AGS cells were determined via western blotting. All western blotting experiments were repeated three times (three panels 1, 2, 3 presents repeated three times). *p < 0.05; **p < 0.01