Fig. 3

ARG1-positive myeloid cells are mainly a Ly6G intermediate population. A Representative flow cytometry plot to visualize ARG1 vs myeloid marker CD11b, in single cells suspension. B Flow cytometry-based quantification of the percentage of ARG1-positive in CD11b⁺ and CD11b⁻ cells (67NR N = 7, 66cl4 N = 8). C Representative flow cytometry plot to visualize the differential frequencies of CD11b vs Ly6G cells. D Flow cytometry-based quantification of the percentages of Ly6G negative, Ly6G positive intermediate and Ly6G positive high cells in the CD11b⁺ subset in 67NR (N = 11) and 66cl4 (N = 13) tumours. E Representative flow cytometry plot to visualize ARG1 vs Ly6G. F Flow cytometry-based quantification as ARG1-positive  cells (% of CD11b⁺ cells) in the three Ly6G populations (67NR N = 8, 66cl4 N = 10)