Fig. 1

Major lipid classes and LD storage in HPDE and PDAC cells. A. TLC images showing major lipid classes in HPDE and PDAC cells cultured in DMEM supplemented with 10% FBS. Band intensity was quantified relative to total lipids of the same sample. Lipid standard mixture (Std.) contains 1 µg for each lipid; B. LD storage in HPDE and PDAC cells cultured in DMEM supplemented with 10% FBS. Cells were stained with BODIPY 493/503 to visualize LDs (green), Phallodin-CF568 to visualize cytoskeleton (red), and Hoechst 33,342 to visualize nuclei (blue); C-D. Quantification of LD content in HPDE and PDAC cells cultured in DMEM, supplemented with 10% or 1% FBS, or 1% FBS plus OA (150 µM), or OA (50 µM) and FC (50 µM). Results are presented as means ± SD (n = 3) in A and means ± 95% confidence interval (n = 30–35 images) in C and D. *p < 0.05 comparing PDAC cells with HPDE cells; ^#p < 0.05 comparing control with OA or OA + FC. CE cholesteryl ester, DAG diacylglycerol, FBS fetal bovine serum, FC free cholesterol, FFA free fatty acids, LD lipid droplet, MAG monoacylglycerol, OA oleic acid, PL phospholipids, TAG triacylglycerol, TLC thin-layer chromatography