Fig. 3

Effect of selected lipid flux inhibitors on PDAC cell migration and invasion. Cells pre-treated with mitomycin C were investigated for A-B. migration and C-D. invasion following their exposure to lipid flux inhibitors (10 µM for HSL/MGLL-i and NCEH1-i, 5 µM for SOAT1-i and HMGCR-i). A. Relative migration distance in 24 h, calculated from the wound-healing assay of confluent cells. B. Representative images for the results in A. C. Cells invaded through Matrigel at 48 h were stained with Hoechst 33342, photographed and quantified. D. Representative images for the results in A. Results are presented as means ± SD (n = 4–8 in A and n = 4 in C, *p < 0.05 comparing inhibitors with DMSO)