Fig. 4

Effect of lipid flux inhibitors, free cholesterol, and fatty acids on PDAC cell density. Cells incubated for 48 h in DMEM supplemented with 1% FBS, with or without OA (100 µM), FC (50 µM) and lipid flux inhibitors (10 µM for HSL/MGLL-i and NCEH1-i, 5 µM for SOAT1-i and HMGCR-i), were assessed for cell density using crystal violet staining. A. Effect of OA and FC on PDAC cell density. B. Combined effect of lipid flux inhibitors and lipids on PDAC cell density. Results are presented as means ± SD (n = 4–5, *p < 0.05 comparing inhibitors with DMSO; ^#p < 0.05 comparing OA or FC treatment with no lipid treatment). FC free cholesterol, OA oleic acid