Table 2 Primer sequences and reaction conditions for genotyping two tag SNPs of RIPK1 gene
SNP | Primer sequence | Ta (℃) | Endonuclease | Product size (bp) |
|---|---|---|---|---|
rs6907943 | F: GTGTTTGTTTGCAGCTCGTTAGCCT | 62 | MnlI | A: 120 |
R: CAGGTGTTGGAGTTCAGCCTGG | C: 33 + 87 | |||
rs9392453 | F: ACAAGCCCCACTTCAGGTTTGGT | 62 | MspI | T: 209 |
R: CTGAACACACAGAAGCACTGGAATCC | C: 27 + 182 |