Fig. 8

RPLP2 silencing promotes ferroptosis of HCC cells. A Gene set enrichment plots of “Oxidative Phosphorylation”, “Regulation of Lipid Catabolic Process”, “Iron Ion Homeostasis” and “WP Ferroptosis” from GSEA of RPLP2-related DEGs. (The data was obtained from TCGA-LIHC). B The correlation between RPLP2 expression and key ferroptosis gene regulators GPX4, IREB2, BECN1 and NCOA4 via FerrDb V2. C The expression levels of RPLP2 and GPX4 in Hep3B cells with RPLP2 knockdown were detected using western blot. D The correlation between RPLP2 and GPX4 protein level was analyzed in 12 clinical HCC tissues. E IHC analysis of the relationship between RPLP2 and GPX4 expression in 12 clinical HCC tissues. F The expression level of RPLP2 in Hep3B cells and HepG2 cells treated with ferroptosis inducer RSL-3 were detected via western blot. G CCK8 assays detected the responses of Hep3B cells knockdown of RPLP2 to Fer-1 and RSL-3. (n = 3 independent experiments). H, I The levels of total GSH (H) and lipid ROS (I) in Hep3B cells knockdown of RPLP2 were detected. (n = 3 independent experiments). ns nonsignificant (P > 0.05), *P < 0.05, **P < 0.01, ***P < 0.001