Fig. 9

Knockdown of RPLP2 inhibits tumor growth of HCC. A Western blot detecting the transfection efficiency of siRPLP2#1 and siRPLP2#2 in the Hep3B cell. B CCK8 assays was applied to detect the effect of RPLP2 knockdown on the cell proliferation of Hep3B cells. (n = 3 independent experiments). C, D The transwell assay of Hep3B cells with RPLP2 knockdown. (n = 3 independent experiments). E, F The effect of RPLP2 knockdown on the colony formation of Hep3B cells was evaluated by colony formation assay. (n = 3 independent experiments). G, H The nude mice xenograft model was established to explore the effect of RPLP2 knockdown on tumor volume (G) and weight (H). (n = 3 mice per group). ns nonsignificant (P > 0.05), *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001