Fig. 5
Macrophage loading with MnMEIO-mPEG nanoparticles. (A) Mouse peritoneal macrophages loaded with NIR-NPs for 1 h at different concentrations and stained with Prussian blue. (B) RAW 264.7 cells loaded with 0.5 µg/ml NIR-NPs at different time points (1 h, 2 h, 3 h, and 4 h). (C) Mouse peritoneal macrophages loaded with NIR-NPs at different concentrations, confocal microscopy. (D) RAW 264.7 cells treated with LPS + IFN-γ + CA2-2 for M1 polarization, and co-cultured with NIR-NPs for 1 h. The cell was stained with DAPI (nucleus, blue color), anti-MHC CLASS II-RPE/Cy5 (M1 marker, biomembrane, green color), and visualized with confocal microscopy. (E) The cytotoxicity effect of NIR-NPs in RAW 264.7 macrophages. The NIR-NPs were incubated with RAW264.7 macrophages at different dose for 24 h. Data are expressed as the means ± SEM (n = 5)