Fig. 7
CA2-2 activated and polarized М1 macrophages recognize the mouse 4T1 tumor. Optical imaging of BALB/cAnN.Cg-Foxn1nu/CrlNarl 4T1 tumor-bearing mice infused by NIR-NPs targeted and CA2-2 stimulated macrophages near the tumor site at different time points (0, 2, 4, 6, 8, and 24 h). The figure shows the front view. Panels (A), (B), (C), (D), (E), and (F) indicate the time points at 0, 2, 4, 6, 8, and 24 h, respectively. a – Represents mice injected with CA2-2 (0.01 µM)-stimulated macrophages. b – Represents mice injected with LPS (10 ng/mL) + IFN-γ (20 ng/mL) + CA2-2 (0.01 µM)-stimulated macrophages. c – Represents mice injected with LPS (10 ng/mL) + IFN-γ (20 ng/mL)-stimulated macrophages. d – Represents mice injected with M0 macrophages. Optical imaging was acquired using an IVIS spectrum system. Emission at 820 nm was measured with an optimal excitation wavelength of 745 nm. Panel (G) Quantification graphic of macrophage-NPs dynamics in tumor over time (h)