Fig. 1
From: Modulation of SLFN11 induces changes in DNA Damage response in breast cancer
Baseline expression of SLFN11 across a panel of 8 breast cancer cell lines and associated methylation profile. A Relative mRNA expression of SLFN11 analyzed by Q-RT-PCR. SLFN11 expression in the different cell lines shown relative to the expression level in HFF (human foreskin fibroblast) used as control cells (N = 6, 3 technical replicates of 2 biological replicates). B Capillary Western blot immunoassay (CWB) of SLFN11 expression in those breast cancer cell lines. Quantification of band intensity of SLFN11 relative to the expression level in HFF cells (N = 4, 2 technical replicates of 2 biological replicates). C Correlation between SLFN11 mRNA analyzed by Q-RT-PCR and protein expression analyzed by CWB. D Percentage of methylation of SLFN11 promoter analyzed by methyl specific PCR (MSP) within the CPG island of the promoter (N = 4, biological replicates). E Correlation of SLFN11 promoter methylation and SLFN11 mRNA expression analyzed by Q-RT-PCR. F Correlation of SLFN11 promoter methylation and SLFN11 protein expression analyzed by CWB