Fig. 2
From: Modulation of SLFN11 induces changes in DNA Damage response in breast cancer
IFN-γ and DAC have limited effect of SLFN11 expression. A, B Relative expression of SLFN11 analyzed by Q-RT-PCR (N = 3, technical replicates) (A) and by CWB (N = 2, technical replicates) (B) after treatment with 5 µM of DAC for 72 h compared to the expression level in untreated HFF. C Percentage of methylation of SLFN11 promoter analyzed by MSP after treatment with 5 µM of DAC for 72 h (N = 4, biological replicates) compared to untreated HFF. D, E Relative expression of SLFN11 analyzed by Q-RT-PCR (N = 3, technical replicates) (D) and by CWB (N = 2, technical replicates) (E) after treatment with 5 nM of IFN-γ for 24 h compared to the expression level in untreated HFF. F Relative mRNA expression of SLFN11 analyzed by Q-RT-PCR after co-culture with CD3/CD28 for 24 h compared to the expression level in HFF (N = 3, technical replicates)