Fig. 3

CRISPR-dCas9 system significantly modulate SLFN11 expression. The UNISAM (unique Synergistic Activation Mediator) system developed by Fidanza et al. was used for CRISPR activation of SLFN11 [14] and KRAB (Krüppel associated box) was used for CRISPR inhibition of SLFN11 [14]. A, B Using the gRNA N7 we could successfully increase (with UNISAM) and decrease (with KRAB) SLFN11 expression in BT-549 as analyzed by Q-RT-PCR (N = 6, 3 technical replicates of 2 biological replicates) (A) and CWB (N = 4, 2 technical replicates of 2 biological replicates) (B). C, D Similarly, Using the gRNA N7 we could successfully increase (with UNISAM) and decrease (with KRAB) SLFN11 expression in T47D as analyzed by Q-RT-PCR (N = 6, 3 technical replicates of 2 biological replicates) (C) and CWB (N = 4, 2 technical replicates of 2 biological replicates) (D). In opposition, the UNISAM and KRAB system used with scramble gRNA (SCR) did not significantly affect SLFN11 expression compared to respective untreated cells (CTRL)