Fig. 6

RNASeq analysis of CRISPR modified cells treated with Cisplatin. A VennDiagram of differentially expressed genes between BT-549 and T47D cell lines in UNISAM up/KRAB down (vs. control), and UNISAM down/KRAB up (vs. control) respectively, (FDR < 0.01, logFC <|1|) in Cisplatin-treated samples. Heatmaps illustrate the expression of DEG genes in UNISAM up/KRAB down and UNISAM down/KRAB up (genes highlighted in red and green in VennDiagram, respectively). B Boxplot of enrichment score generated for list of DEG (UNISAM up/KRAB down (n = 94) and UNISAM down/KRAB up (n = 87)) in BT-549 and T47D cell lines. Statistical significance was assessed using an unpaired t test (C). Dotted heatmap of mean enrichment score for apoptosis and glycogen metabolism pathways. D Boxplot of enrichment scores for selected apoptosis and glycogen metabolism pathways in BT-549 and T47D cell lines Statistical significance was assessed using an unpaired t test. BT-549 CTRL and modified cells (UNISAM and KRAB) N = 6. T47D CTRL and modified cells (UNISAM and KRAB) N = 5. Cisplatin treated samples for each cell line N = 9 for all analysis performed