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Fig. 2 | Cancer Cell International

Fig. 2

From: DHRS4-AS1 regulate gastric cancer apoptosis and cell proliferation by destabilizing DHX9 and inhibited the association between DHX9 and ILF3

Fig. 2

LncRNA DHRS4-AS1 promotes gastric cancer cell apoptosis and inhibits proliferation in vitro. (A) Relative DHRS4-AS1 expression was evaluated after transfection with DHRS4-AS1 Lentivirus. (B and C) EdU and clone formation assays to assess DHRS4-AS1-overexpressing GC cell proliferation. (D) Cell apoptosis was determined by flow cytometry after DHRS4-AS1 overexpressed. (E) Western blot assay of Bax and Bcl-2 expression in GC cells overexpressing DHRS4-AS1. (F) Relative DHRS4-AS1 expression was measured to evaluate transfection efficiency. (G and H) Clone formation assay and EdU assays were used to measure GC cell proliferation after transfection with DHRS4-AS1 knock-down plasmid. (I) Cell apoptosis was determined by flow cytometry after DHRS4-AS1 knockdown. (J) Western blot assays to evaluate Bax and Bcl-2 expression in GC cells after DHRS4-AS1 knockdown. *p < 0.05, **p < 0.01, and ***p < 0.001

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Cancer Cell International

ISSN: 1475-2867

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