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Fig. 5 | Cancer Cell International

Fig. 5

From: DHRS4-AS1 regulate gastric cancer apoptosis and cell proliferation by destabilizing DHX9 and inhibited the association between DHX9 and ILF3

Fig. 5

LncRNA DHRS4-AS1 promotes interactions between DHX9 and the E3 ligase MDM2. (A) Candidate E3 ligases that interact with DHX9 were predicted from the Human Protein Reference Database. (B) Immunoblotting following immunoprecipitation with an DHX9 antibody verified that MDM2, rather than SYVN1, interacts with DHX9. (C) DHRS4-AS1 pull-down followed by western blotting validated interactions with MDM2. (D) Immunostaining of DHX9 and MDM2 in AGS and HGC-27 cells. (E) Immunoblotting for DHX9 and MDM2 following immunoprecipitation from AGS cells from the Sh-NC, Sh-DHRS4-AS1#1, and Sh-DHRS4-AS1#2 groups. (F) Immunoblotting for DHX9 and MDM2 following immunoprecipitation in vector and DHRS4-AS1-overexpressing HGC-27 cells

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Cancer Cell International

ISSN: 1475-2867

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