Fig. 6

DHX9 is upregulated in GC and is inhibited by lncRNA DHRS4-AS1. (A) Immunohistochemistry was performed to detect DHX9 expression in gastric cancer and adjacent normal tissues. (B) Western blotting was used to detected DHX9 protein expression in gastric cancer and adjacent normal tissues. (C) Relative DHX9 expression in GC cells after transfection Sh-DHX9-NC, Sh-DHX9-1, and Sh-DHX9-2. (D and E) CCK-8 and clone formation assays to examine DHX9-transfected cell proliferation. (F) FCM of apoptosis in GC cells transfected with DHX9. (G) Relative DHX9 expression level was measured using western blotting in cells transfected with empty vector and DHRS4-AS1 overexpression groups. (H) CCK-8 assay measuring the proliferation of transfected cells. (I) Colony formation assay to examine cell proliferation after DHX9 knockdown. (J) FCM assay to examine apoptosis in transfected cells. *p < 0.05, **p < 0.01 NS:Not significant