Fig. 7

lncRNA DHRS4-AS1 inhibited the associate between DHX9 and ILF3. (A) Western blot analysis of co-IPs performed in AGS and HGC-27 with DHX9 and ILF3 antibody. (B) Western blot analysis of co-IPs performed on lysates prepared from HEK293 cells transfected with ILF3-Flag alone or together with indicated DHX9-Myc constructs. Top, co-IP performed with anti-Myc; bottom, input protein. (C) Western blot analysis of co-IPs performed on lysates prepared from HEK293 cells transfected with DHX9-Myc alone or together with indicated ILF3-Flag constructs. Top, CO-IP performed with anti-Myc; bottom, input protein. (D) The correlation between DHX9 and ILF3 was analysed in ChipBase V2.0 database. (E) Western blot analysis was performed to detected the expression of ILF3 in DHX9 overexpressd and knockdown GC cell. (F) Overexpression of DHX9 activate the phosphorylation of NF-κB (P65) and IκBα on protein level while was seriously alleviated by ILF3 knockdown. (G) Western blot analysis of co-IPs performed with anti-DHX9 and lysates prepared from DHRS4-AS1 overexpressd and knockdown GC cells. Cells were pretreated with MG132 (5 µM) for 90 min.IgG groups served as negative controls