Fig. 2

Characterization of CAR-TIM3 iPSCs. A Representative micrograph of CAR-TIM3 iPSC colony. B Expression of NANOG, OCT4, and SOX2 transcripts as quantitative measurement by qPCR analysis. Data are mean ± SEM (n = 3). *p < 0.05 versus H1 ESC; unpaired t-test. C Representative micrographs of immunofluorescence staining showing pluripotency markers OCT4, NANOG, and SOX2 proteins. D Flow cytometric analysis of SSEA-3, SSEA-4, TRA-1-60 and TRA-1-81 positive cells. E Karyotypic analysis as determined by G-banding assay. F Evidence of the loss of episomal transgenes detected by PCR and gel electrophoresis. G STR analysis comparing a total of 16 loci between starting UCB-NK cells, MUSIi013-A NK/iPSCs and CAR-TIM3 iPSCs