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Fig. 3 | Cancer Cell International

Fig. 3

From: Anti-TIM3 chimeric antigen receptor-natural killer cells from engineered induced pluripotent stem cells effectively target acute myeloid leukemia cells

Fig. 3

Differentiation potential of MUSIi013-A NK/iPSCs and its subclone CAR-TIM3 iPSCs. A Differentiation potential of parental MUSIi013-A NK/iPSCs was first performed by teratoma formation in SCID mice. Representative micrographs of H&E stained sections of ectoderm, mesoderm, and endoderm. B–D Differentiation potential of CAR-TIM3 iPSCs as evaluated by EB formation. Morphology of floating EBs on days 4, 7 and 14 of differentiation. C mRNA expression of tri-lineage differentiation markers: PAX6, OTX1, and MAP2 for ectoderm; TBX6, HAND1, and NKX2.5 for mesoderm; and LEFTY1 and AFP for endoderm. Data are mean ± SEM (n = 3). **p < 0.01, ***p < 0.001 versus non-differentiated CAR-TIM3 iPSCs; unpaired t-test. D Representative micrographs of immunofluorescence staining of tri-lineage differentiation markers

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Cancer Cell International

ISSN: 1475-2867

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