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Fig. 4 | Cancer Cell International

Fig. 4

From: Anti-TIM3 chimeric antigen receptor-natural killer cells from engineered induced pluripotent stem cells effectively target acute myeloid leukemia cells

Fig. 4

Generation of spin EBs and induction of HSPCs from CAR-TIM3 iPSCs. A Schematic diagram illustrating a two-stage protocol for the differentiation of NK cells from iPSCs under serum- and feeder-free conditions. B Morphology of generated EBs on various days of culture. iPS sacs were observed on day 12 onwards and completely disappeared on day 28. C, D Representative flow cytometric analysis of HSPC markers CD34, CD43, and CD45 in floating cells on days 12, 16, and 20 of culture

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